import openpyxl workbook = openpyxl.load_workbook("/path/to/workbook.xlsx") worksheet = workbook.active for row in worksheet.iter_rows(min_row=2, min_col=1, max_col=3): value1 = row[0].value value2 = row[1].value value3 = row[2].value # Process values workbook.save("/path/to/workbook.xlsx")